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[Technology] (Basic Principle) Proteolytic enzymes?
작성자 : 관리자(bioms@hanmail.net)  작성일 : 2020.07.22   조회수 : 220

In the living organism, proteolytic enzymes (proteases) are produced to degrade and modify proteins. A main task for proteolytic enzymes is to degrade proteins into peptides or amino acids to be used either as an energy source or as building blocks for resynthesis of proteins. Furthermore, proteolytic enzymes modify cellular environments and facilitate cell migration in connection with wound repair and cancer, ovulation and implantation of the fertilized egg, embryonic morphogenesis, and involution of mammary glands after lactation.
Another important function of the proteases is their role as regulators in processes such as inflammation, infection and blood clotting. Most proteolytic enzymes are highly specific for their substrates. The classification of proteases, however, is not based on their choice of substrate but on their mechanism of action.
Four different groups of proteolytic enzymes, named after the active site amino acid residue responsible for the catalytic activity, are generally distinguished: the aspartic proteases (e.g. pepsin), the cystein proteases (e.g. cathepsin B and cathepsin H), the serine proteases (e.g. trypsin, thrombin and plasmin) and metalloproteases (e.g. collagenases and gelatinases).Although the members of each group of proteolytic enzymes may have very diverse biological functions, amino acid analysis often shows a high degree of structural similarity between them. Detailed knowledge of the structure and mechanism of action of one enzyme can in many cases reveal an understanding of the structure and functions of other enzymes within the same group.

 

 

Classes of proteases

 Name  active site 
 serine proteases  Ser His Asp*
 cystein
proteases
 Cys His Asp*
 aspartic proteases  Asp Asp
 metallo proteases  His His Zn2+

 

*Asp not always present

이전글 (Basic Principle) Chromogenic Substrates란?
다음글 (Basic Principle) Serine proteases?